Hepg2 stable cell line generation and rnai applications sirna transfection. Effects of estrogen and estrogen receptors on transcriptomes. Given the there are many formulations of dmem and different versions of serum, we would like to provide the catalog numbers of. The parental cells were stably transfected at passage 48 with a human cholesterol 7 alphahydroxylase cyp7 minigeneluciferase construct. However, p53 was neither upregulated, nor showed any evidence of complexing with dengue virus proteins as determined by immunoprecipitation. The expression of pd1 was significantly increased and cytotoxicity of nk92 cells was significantly decreased by the dexamethasone treatment only in the coculture system with the presents of hepg2 cells but no changes were shown in the independent culture of nk92 cells figure 5ch, which indicated that the increase of pd1 expression was.
Human hepatocellular carcinoma cell line hepg2, human acute. Pediatric hepatocellular carcinoma is a rare tumor associated with much worse prognosis and aggressive behavior than hb and significantly less responsive to. After infection, a p53 wild type liver cell line hepg2 showed changes consistent with apoptosis including alterations of cell morphology, cellular detachment and dna laddering. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services.
I seeded them at 1x106100mm plate, and subculture in about 5 days without changing media during culture. Fibroblast growth factor 9 is expressed by activated. Later, we conducted in vitro experiments and observed changes in hepg2 cell cycle after the administration of quercetin. Hepg2 is an immortalized cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15yearold caucasian male who had a welldifferentiated hepatocellular carcinoma, which is the fifth mostcommon cancer worldwide. The mice were anesthetized and the liver was exposed as mentioned above.
Antioxidant activity of oat proteins derived peptides in. Fibroblast growth factor 9 is expressed by activated hepatic. Hello, im going to culture hepg2 cells soon, i studied many research articles but. Nevirapine nvp is an alkyldiarylamine, containing two aryl and one alkyl groups attached to an amino group 1 that is commonly used for the treatment of patients with. The human colorectal cancer sw480 cells ccl228 were purchased from the american type culture collection atcc, manassas, va, usa. Thank you for your understanding, patience and flexibility as atcc does everything it can to help reduce the impact of the coronavirus pandemic to our valued customers. Human hepg2 hepatoma cells are highly permissive for influenza virus type a and type b, even without the addition of trypsin, and they exhibit a marked cytopathic effect.
Effect of shikonin on multidrug resistance in hepg2. Therefore, the focus of this study was the in vivo anticancer effects of quercetin on hepg2 tumor bearing animal model. C3a hepg2c3a, derivative of hep g2 atcc hb8065 atcc crl10741. Cultures of primary hepatocytes and hepatoma cell line hepg2 are frequently used in in vitro models for human biotransformation studies. Atcc catalog fy 2020 atcc catalog pdf a hard copy of this catalog may be requested by contacting the information center at 3207624600 or email information center. The cells express 3hydroxy3 methylglutarylcoa reductase and hepatic triglyceride lipase activities.
Human hepatocellular carcinoma cell line hepg2, human acute monocytic leukemia cells thp1, and human normal lung cells wi38 were purchased from the american type culture collection atcc, rockville, md, usa and cultured as recommended by the atcc. The hepg2 cell line is one of the most widely used cancer cell lines used in many areas of. This property greatly facilitates the primary isolation of influenza viruses. In this study, we characterized and compared the capacity of these model systems to indicate the presence of different classes of promutagens. High permissivity of human hepg2 hepatoma cells for influenza. This list shows national collection of type cultures nctc bacterial strain equivalents to american type culture collection atcc bacterial strains. To atcc valued customers, atcc stands ready to support our customers needs during the coronavirus pandemic.
Hepatic stellate cells hsc and cancerassociated myofibroblasts are key players in liver fibrogenesis and. In addition, the low expression of cyclin d1 was observed. I want do some research about the influence of hbv for hepatocellular carcinoma. Methods hct116 and hepg2 cells were treated with mphx for 24 hr. Nevirapine induced mitochondrial dysfunction in hepg2. Atcc recommended not to hit or shake the cells during trypsinization, but i did do so to detach the cells. Hepatocellular carcinoma hcc is closely associated with liver fibrosis. Mar 11, 2020 hepatocellular carcinoma hcc is closely associated with liver fibrosis. Investigation into the effects of antioxidantrich extract of. In these cells, the hbv genome is integrated in several sites of hepg2 cellular dna. The objective of this study was to investigate the effect of lactobacillus acidophilus atcc 4356 on the development of atherosclerosis in apolipoprotein eknockout apoe.
You may not be able to if you cant get it from the atcc try asking authors from some of the papers that use the line. Apoptotic and inhibitory effects on cell proliferation of. The role of sirt1 yongdong jin 1, yi ren 2, mingwei wu 1, ping chen 1, and jin lu 1 1 department of medical oncology, sichuan cancer hospital, chengdu, china and 2 department of clinical medicine, medical college. We also compiled a phased crispr map of loci suitable for allele specifictargeted genome editing or screening. We constructed a new plasmid, psehflaghbv, and transfected it into hepg2 cells, and.
Nctc number current name type strain atcc number nctc 10802 actinobacillus rossii no atcc 27073. Hepg2 cells were seeded and pretreated with the ic20 concentration of the plant extracts. Microarray gene expression profiling in colorectal hct116. Aug 23, 2017 nevirapine nvp is an alkyldiarylamine, containing two aryl and one alkyl groups attached to an amino group 1 that is commonly used for the treatment of patients with acquired immunodeficiency.
Atcc s collections include a wide range of biological materials for research, including cell lines, molecular genomics tools, microorganisms, and bioproducts. Exosomes derived from sw480 colorectal cancer cells promote. The human hepatocellular cancer hepg2 cells jcrb1054 were purchased from the health science research resources bank osaka, japan. This list shows national collection of type cultures nctc. Thepredatorybacteriausedin thestudy werebdellovibriobacteriovorus strains 109jatcc1 43826and hd100atcc 1 1535615,and micavibrio aeruginosavorus strain arl 8. Growth medium for hepg2 dmem 10% fbs penstrep 1x procedure a. Stable expression and integrated hepatitis b virus genome in. Media is mem with 10% fbs and other stuff recommended, including ps. Following the pretreatment with the extracts, hepg2 cells were exposed to oxidative stress condition by induction with 1 mm of h2o2 for 2 h. Receipt of frozen cells and starting cell culture 1 immediately place frozen cells in liquid nitrogen freezer storage until ready to culture. Mitochondrial function was analyzed using western blotting, immunofluorescence assay. As the cells become confluent, there is a marked reduction in afp secretion. Atcc stands ready to support our customers needs during the coronavirus pandemic.
To the editor, hepatoblastoma hb is an embryonal malignancy of hepatocellular origin and the most common primary liver tumor of childhood, often presenting in the first years of life 1. Apoptosis in dengue virus infected liver cell lines hepg2 and. Full text genetic ablation of taz induces hepg2 liver. Hepg2 cells were maintained in phenol redfree dmem supplemented with 10% vv heatinactivated and charcoalstripped fbs, 1% antibiotics of 50 uml. Intracellular glutathione regulates andrographolideinduced cytotoxicity on hepatoma. While we are not currently experiencing delays due to this pandemic, we expect that we could see them as the situation evolves. Genotoxic sensitivity, enzyme activity, and gene expression were monitored in response to treatment with food. Epigenetic modifications of the liver tumor cell line hepg2. Total rna was extracted from the cells and used for transcriptome profiling using applied biosystem genechip human gene 2. What are different variant insertion gene editing techniques used for human.
Haplotyperesolved and integrated genome analysis of the cancer. The aim of our study was to investigate the influence of taz on hepg2 liver cancer cell apoptosis. These multiple copies may have some influence on the cellular processes. This is the complete description of the cell line that you selected. What is the most efficient method to culture hepg2 cells. Hepg2 cells were maintained in phenol redfree dmem supplemented with 10% vv heatinactivated and charcoalstripped fbs. In addition to the hepatocellular carcinoma or hepatoma cell line based on the original publication pubmed.
There is no evidence of a hepatitis b virus genome in this cell line. After 24 h, the cells were subsequently washed with icecold pbs and detached using a scraper. This has been corrected in both the pdf and html versions of the article. Maintaining healthy cells is the key to experimental success and reproducible research results. Cell line was derived from the hepatocellular carcinoma cell line, hepg2 atcc hb8065. Costus speciosus is a medicinal plant commonly known as wild ginger distributed in south and southeast asian countries. Propagation of hepg2 2 5 immediately remove cells and pellet at 500 xg for 5 minutes 4oc. I have followed all the steps shown in the atcc manual for the hepg2 cell line. The cells were injected into the left lobe of liver of ten nude mice.
Investigation of the anticancer effect of quercetin on hepg2. Leaves of this plant are used for ayurvedic treatment regimes in malignancies and mental illness. The cell line hepg2 shows the highest similarity in its epigenetic profile compared to phh was used for further testing. Hepg2 liver hepatocellular carcinoma cell culture and. C3a hepg2c3a, derivative of hep g2 atcc hb8065 atcc.
Rhizome extract from the plant is used to treat malignancies, pneumonia, urinary disorders, jaundice, rheumatism, and diabetes. Gene expression data was analysed using an applied biosystems expression console and transcriptome analysis console software. Hepg2 liver cancer cell was used in the present study, and shrna against taz was transfected into hepg2 cell to knockdown taz expression. The mistaken classification of the hep g2 tumor of origin has created confusion between investigators and a divided. Every step of the way, a wide range of cell health products. Hepg2, a human hccderived cell line, was originally obtained from the american type culture collection atcc, manassas, va, usa and authenticated using short tandem repeat analysis by atcc. Hepg2 liver hepatocellular cells description, culturing medium conditions, transfection efficiency and protocols, and optimized transfection kits. Atcc has the experience and capabilities to provide standard testing and custom services to support your research, development and production projects. Established from the tumor tissue of an 8yearold black boy species.
547 145 306 923 1493 1459 967 1555 31 87 280 366 1416 1287 206 895 188 1126 877 110 1534 115 845 401 997 959 535 880 162 363 1490 865 1082 887 406